ERI1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Exoribonuclease 1

Gene Aliases

3' exoribonuclease;3'-5' exonuclease ERI1;3'-5' exoribonuclease 1;3'HEXO; enhanced RNAi three prime mRNA exonuclease homolog 1; Eri-1 homolog; HEXO; histone mRNA 3' end-specific exonuclease; histone mRNA 3'-end-specific exoribonuclease; histone mRNA 3'-exonuclease 1; Protein 3'hExo; THEX1; three prime histone mRNA exonuclease 1; three prime mRNA exonuclease 1.

Gene ID

90459

Accession Number

NP_699163.2

Reactivity

Human|Porcine

Immunogen

Range: Glu2 - Lys249

Target

RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi) . Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Molecular Weight

40 kDa

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against ERI1. It has been selected for its ability to recognize ERI1 in immunohistochemical staining and western blotting.