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SPC25 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

SPC25 component of NDC80 kinetochore complex

Gene Aliases

2600017H08Rik; AD024; hSpc25; kinetochore protein Spc25; SPBC25; SPC25, NDC80 kinetochore complex component, homolog; spindle pole body component 25 homolog.

Gene ID

57405

Accession Number

NP_065726

Reactivity

Human

Immunogen

Recombinant Human Kinetochore protein Spc25 protein (1-224AA)

Target

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735) . Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129) . The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunohistochemistry

Purification

Protein G Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid

Reconstitution

-20°C or -80°C

Fragment

IgG

NCBI Gene Symbol

SPC25

Host or Source

Rabbit

Protein Name

Kinetochore protein Spc25

Gene Name URL

SPC25

Nucleotide Accession Number

NM_020675

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