CHCHD4 Antibody
Product Specifications
Gene Name
Coiled-coil-helix-coiled-coil-helix domain containing 4
Gene Aliases
Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; MIA40; mitochondrial intermembrane space import and assembly protein 40; TIMM40; translocase of inner mitochondrial membrane 40 homolog.
Gene ID
131474
Accession Number
NP_001091972
Reactivity
Human
Immunogen
Recombinant Human Mitochondrial intermembrane space import and assembly protein 40 protein (1-142AA)
Target
Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364, PubMed:23676665) . Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS) . Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23676665) . Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364) . Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunohistochemistry
Purification
Protein G Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Format
Liquid
Reconstitution
-20°C or -80°C
Fragment
IgG
NCBI Gene Symbol
CHCHD4
Host or Source
Rabbit
Protein Name
Mitochondrial intermembrane space import and assembly protein 40
Gene Name URL
CHCHD4
CAS Number
9007-83-4
Nucleotide Accession Number
NM_001098502
Curated Selection
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