KDM8 Antibody

Arginyl C3-hydroxylase KDM8; bifunctional peptidase and arginyl-hydroxylase JMJD5; JmjC arginyl-hydroxylase; jmjC domain-containing protein 5; JMJD5; jumonji C domain-containing protein 5; jumonji domain containing 5; jumonji domain-containing protein 5; L-arginine (3R) -hydroxylase KDM8; lysine (K) -specific demethylase 8; lysine-specific demethylase 8.

Histone demethylase required for G2/M phase cell cycle progression (PubMed:20457893) . Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator (PubMed:20457893) . Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation (PubMed:20457893) . In a complex with coregulator RCCD1, is also able to demethylate trimethylated 'Lys-36' (H3K36me3) of histone H3 (PubMed:24981860) . Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1 (PubMed:24981860) . Possibly together with RCCD1, involved in proper mitotic spindle organization and chromosome segregation (PubMed:24981860) . Plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability (PubMed:28455245) . Might function as a protein hydroxylase (PubMed:22851697, PubMed:24100311) . Under stress conditions that cause a DNA damage response, acts as a histone protease by cleaving the N-terminal tail of histone H3 at the carboxyl side of monomethyl-lysine (Kme1) residues, preferably at monomethylated 'Lys-9' (H3K9me1) (PubMed:28982940) . The histone variant H3F3A is the major target for cleavage (PubMed:28982940) .

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol