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CASP3 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Caspase 3

Gene Aliases

CPP32, SCA-1, CPP32B

Gene ID

836

Swiss Prot

P42574

Accession Number

NP_004337.2

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human CASP3.

Target

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1) . Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

WB, IHC, IF

Purification

Affinity purification.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid.// PBS with 0.02% sodium azide, 50% glycerol, pH 7.3

Reconstitution

Store at -20C. Avoid repeated freeze/thaw cycles.

Molecular Weight

32 kDa

Fragment

IgG

NCBI Gene Symbol

CASP3

Host or Source

Rabbit

Protein Name

Caspase-3

Gene Name URL

CASP3

Nucleotide Accession Number

NM_004346.3

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