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ACTB Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Actin, beta

Gene Aliases

BRWS1, PS1TP5BP1

Gene ID

60

Swiss Prot

P60709

Reactivity

Human, Mouse, Rat, Pig, Zebrafish, Bovine, Sheep, Rabbit, Goat, Guinea Pig, Dog, Monkey, Hamster, Chicken, Plants, Rice, Fish

Immunogen

Full-length beta-Actin protein of human

Target

Beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Clonality

Monoclonal

Type

Monoclonal Antibody

Applications

WB, IHC, IF, ICC

Purification

Affinity-chromatography

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid// PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol2+2+

Reconstitution

Store at -20° C. Stable for 12 months from date of receipt.

Molecular Weight

43 kDa

Fragment

IgG

Specificity

Beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.

NCBI Gene Symbol

ACTB

Host or Source

Mouse

Gene Name URL

ACTB

Frequently Asked Questions

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