ACTB Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Actin, beta
Gene Aliases
BRWS1, PS1TP5BP1
Gene ID
60
Swiss Prot
P60709
Reactivity
Human, Mouse, Rat, Pig, Zebrafish, Bovine, Sheep, Rabbit, Goat, Guinea Pig, Dog, Monkey, Hamster, Chicken, Plants, Rice, Fish
Immunogen
Full-length beta-Actin protein of human
Target
Beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Clonality
Monoclonal
Type
Monoclonal Antibody
Applications
WB, IHC, IF, ICC
Purification
Affinity-chromatography
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid// PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol2+2+
Reconstitution
Store at -20° C. Stable for 12 months from date of receipt.
Molecular Weight
43 kDa
Fragment
IgG
Specificity
Beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.
NCBI Gene Symbol
ACTB
Host or Source
Mouse
Gene Name URL
ACTB
Frequently Asked Questions
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