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PRKAB1 Antibody (Phospho-Ser181)

Product Specifications

CAS Number

9007-83-4

Gene Name

Protein kinase, AMP-activated, beta 1 non-catalytic subunit

Gene Aliases

AMPK, HAMPKb

Gene ID

5564

Swiss Prot

Q9Y478

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human AMPK beta-1 around the phosphorylation site of Serine 181

Target

AMPK-beta-1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPK-beta-1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, IF, ICC

Purification

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid// PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

Store at -20° C. Stable for 12 months from date of receipt.

Molecular Weight

38 kDa

Fragment

IgG

Specificity

Phospho-AMPK beta-1 (Ser181) antibody detects endogenous levels of AMPK beta-1 only when phosphorylated at Serine 181

NCBI Gene Symbol

PRKAB1

Host or Source

Rabbit

Gene Name URL

PRKAB1

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