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SIRT1 Antibody (Phospho-Ser47)

Product Specifications

CAS Number

9007-83-4

Gene Name

Sirtuin 1

Gene Aliases

SIR2, SIR2L1, SIR2alpha

Gene ID

23411

Swiss Prot

Q96EB6

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human SirT1 around the phosphorylation site of Serine 47

Target

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB

Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

Stable at -20° C for at least 1 year.

Molecular Weight

110 kDa

Fragment

IgG

Specificity

Phospho-SirT1 (Ser47) antibody detects endogenous levels of SirT1 only when phosphorylated at Serine 47.

NCBI Gene Symbol

SIRT1

Host or Source

Rabbit

Gene Name URL

SIRT1

Frequently Asked Questions

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