SMAD3 Antibody (Phospho-Thr8)
Product Specifications
CAS Number
9007-83-4
Gene Name
SMAD family member 3
Gene Aliases
LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436
Gene ID
4088
Swiss Prot
P84022
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Smad2/3 around the phosphorylation site of Threonine 8
Target
Smad2 ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IHC, P-ELISA
Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid//PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
Reconstitution
Store at -20C. Stable for 12 months from date of receipt.
Molecular Weight
48 kDa, 60 kDa
Specificity
Phospho-Smad2/3 (Thr8) antibody detects endogenous levels of Smad2/3 only when phosphorylated at Threonine 8
NCBI Gene Symbol
SMAD3
Host or Source
Rabbit
Gene Name URL
SMAD3
Curated Selection
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