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SMAD3 Antibody (Phospho-Thr8)

Product Specifications

CAS Number

9007-83-4

Gene Name

SMAD family member 3

Gene Aliases

LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436

Gene ID

4088

Swiss Prot

P84022

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Smad2/3 around the phosphorylation site of Threonine 8

Target

Smad2 ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, P-ELISA

Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid//PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol

Reconstitution

Store at -20C. Stable for 12 months from date of receipt.

Molecular Weight

48 kDa, 60 kDa

Specificity

Phospho-Smad2/3 (Thr8) antibody detects endogenous levels of Smad2/3 only when phosphorylated at Threonine 8

NCBI Gene Symbol

SMAD3

Host or Source

Rabbit

Gene Name URL

SMAD3

Curated Selection

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