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PLCG1 Antibody (Phospho-Tyr771)

Product Specifications

CAS Number

9007-83-4

Gene Name

Phospholipase C, gamma 1

Gene Aliases

PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1

Gene ID

5335

Swiss Prot

P19174

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyrosine 771

Target

The protein encoded by this gene catalyzes the formation of inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor) -activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

Immunogen affinity purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

Store at -20C. Stable for 12 months from date of receipt.

Molecular Weight

155 kDa

Specificity

Phospho-PLCG1 (Tyr771) antibody detects endogenous levels of PLCG1 only when phosphorylated at Tyrosine 771

NCBI Gene Symbol

PLCG1

Host or Source

Rabbit

Gene Name URL

PLCG1

Frequently Asked Questions

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