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RELA Antibody (Phospho-Ser536)

Product Specifications

CAS Number

9007-83-4

Gene Name

V-rel avian reticuloendotheliosis viral oncogene homolog A

Gene Aliases

P65, CMCU, NFKB3

Gene ID

5970

Swiss Prot

Q04206

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Serine 536.

Target

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1) /p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC, IP, IF

Purification

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho-peptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid.// PBS, pH 7.4, 150mM NaCl, sodium azide and glycerol

Reconstitution

Store at -20° C. Stable for 12 months from date of receipt.

Molecular Weight

65 kDa

Fragment

IgG

Specificity

Phospho-NF- kappaB p65 (Ser536) Ab detects endogenous levels of NF- kappaB p65 only when phosphorylated at Serine 536.

NCBI Gene Symbol

RELA

Host or Source

Rabbit

Gene Name URL

RELA

Frequently Asked Questions

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