MAPKAPK5 Antibody (Phospho-Thr182)
Product Specifications
CAS Number
9007-83-4
Gene Name
Mitogen-activated protein kinase-activated protein kinase 5
Gene Aliases
MK5, MK-5, PRAK, MAPKAP-K5
Gene ID
8550
Swiss Prot
Q8IW41
Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Threonine 182
Target
The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IHC, IF, ICC
Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid//PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol2+2+
Reconstitution
Store at -20C. Stable for 12 months from date of receipt.
Molecular Weight
60 kDa
Fragment
IgG
Specificity
Phospho-MAPKAPK5 (Thr182) antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Threonine 182
NCBI Gene Symbol
MAPKAPK5
Host or Source
Rabbit
Gene Name URL
MAPKAPK5
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