NOS3 Antibody (Phospho-Ser615)
Product Specifications
CAS Number
9007-83-4
Gene Name
Nitric oxide synthase 3 (endothelial cell)
Gene Aliases
ENOS, ECNOS
Gene ID
4846
Swiss Prot
P29474
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human eNOS around the phosphorylation site of Sersine 615
Target
ENOS is an endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IF/ICC_x005F _x005F
Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid.// PBS pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reconstitution
Store at -20C. Stable for 12 months from date of receipt.
Molecular Weight
140 kDa
Fragment
IgG
Specificity
Phospho-eNOS (Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at Sersine 615
NCBI Gene Symbol
NOS3
Host or Source
Rabbit
Gene Name URL
NOS3
Curated Selection
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