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NOS3 Antibody (Phospho-Ser615)

Product Specifications

CAS Number

9007-83-4

Gene Name

Nitric oxide synthase 3 (endothelial cell)

Gene Aliases

ENOS, ECNOS

Gene ID

4846

Swiss Prot

P29474

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human eNOS around the phosphorylation site of Sersine 615

Target

ENOS is an endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IF/ICC_x005F _x005F

Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid.// PBS pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

Store at -20C. Stable for 12 months from date of receipt.

Molecular Weight

140 kDa

Fragment

IgG

Specificity

Phospho-eNOS (Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at Sersine 615

NCBI Gene Symbol

NOS3

Host or Source

Rabbit

Gene Name URL

NOS3

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