DDX4 Antibody (Phospho-Ser43)

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; probable ATP-dependent RNA helicase DDX4; VASA; vasa homolog.

A synthetic peptide corresponding to the epitope -GGSGG- with the phosphorylated serine of human VASA protein. This sequence is identical within human, rat, mouse.

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebRate and vertebRate species is restricted to germ cells and is required for gemr cell formation. VASA is therefore a highly specific marker of germ cells. This protein is located in cytoplasmic and is present only in migRatory primordial germ cells in the region of the gonadal ridge. On testicular sections, VASA expression is the highest in spermatogonia, reduced in spermatocytes, low in spermatids and absent in sperm. In the ovary, VASA expression is the highest in oogonia but persists throμ ghout oogenesis. VASA has a glycine-rich N-terminus with multiple repeats of an RGG motif believed to function in RNA binding. The phosphorylation of VASA at N-terminus may regulate the translation of intricate mRNAs that are essential for differentiation.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.