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Anti-His Tag Polyclonal Antibody

Product Specifications

CAS Number

9007-83-4

Gene Aliases

HHHHHH

Reactivity

Tag antibody

Immunogen

A synthetic peptide of HHHHHH conjugated to a carrier protein.

Target

Many oligo peptides or tag proteins are widely used as a tag to monitor the protein expression, such as FLAG, c-Myc, E-tag, His-tag, GFP, GST, and etc. These tag antibodies provide tools to localize gene products in a variety of cell types to study the topology of proteins, and protein complexes, and to identify associated proteins. In addition, this Antibody allows characterizing of newly identified, lowing abundance or poorly immunogenic proteins when protein specific antibodies are not available. The His tag Antibody is geneRated according to the 6 amino acid epitope tag (HHHHHH), and it can be used to detect the His tagged fusion proteins.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

100ug/200ul

Format

Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

Reconstitution

The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Molecular Weight

0.8

Fragment

Rabbit IgG

Host or Source

Rabbit

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