Anti-Osteopontin Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Secreted phosphoprotein 1

Gene Aliases

BNSP; BSPI; early T-lymphocyte activation 1; ETA-1; nephropontin; OPN; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) ; secreted phosphoprotein 1 variant 6; SPP1/CALPHA1 fusion; urinary stone protein; uropontin.

Gene ID

6696

Swiss Prot

P10451

Reactivity

Human|Mouse|Rat

Immunogen

A synthetic peptide corresponding to the internal portion of Osteopontin protein sequence. This sequence is identical to human, rat and mouse origins.

Target

Osteopontin (OPN or Early T-Lymphocyte Activation-1: Eta-1), also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, and minopotin, is a highly phosphorylated and glycosylated phosphoprtein that is expressed in many tissues. It is acidic, calcium-binding, cell surface-binding sequences (RGD) -containing protein was originally isolated from bone matrix. Osteopontin has been found in kidney, placenta, blood vessels. It is over-expressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

Reconstitution

The antibodies are stable for 24 months from date of receipt when stored at -20° C to -70° C. The antibodies can be stored at 2-8° C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.