TOP2A Antibody (Phospho-Ser1525)
Product Specifications
CAS Number
9007-83-4
Gene Name
DNA topoisomerase II alpha
Gene Aliases
DNA gyrase; DNA topoisomerase (ATP-hydrolyzing) ; DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; TOP2alpha; TOPIIA; topoisomerase (DNA) II alpha 170kDa; TP2A.
Gene ID
7153
Swiss Prot
P11388
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human TOP2A around the phosphorylation site of Ser1525.
Target
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
VSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
174 kDa
Notes
Formerly GWB-ASC019
Specificity
TOP2A (Phospho-Ser1525) Antibody detects endogenous levels of TOP2A only when phosphorylated at Ser1525.
NCBI Gene Symbol
TOP2A
Host or Source
Rabbit
Protein Name
DNA topoisomerase 2-alpha
Gene Name URL
TOP2A
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
