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CREB Antibody (Phospho-Ser129)

Product Specifications

CAS Number

9007-83-4

Gene Name

CAMP responsive element binding protein 1

Gene Aliases

Active transcription factor CREB; cAMP-response element-binding protein-1; CREB; CREB-1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; transactivator protein.

Gene ID

1385

Swiss Prot

P16220

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser129.

Target

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

LFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDA

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Reconstitution

-20°C

Molecular Weight

36 kda

Notes

Formerly GWB-ASB641

Specificity

CREB (Phospho-Ser129) Antibody detects endogenous levels of CREB only when phosphorylated at Ser129.

NCBI Gene Symbol

CREB1

Host or Source

Rabbit

Protein Name

Cyclic AMP-responsive element-binding protein 1

Gene Name URL

CREB1

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