CDC25C Antibody (Phospho-Ser216)
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Product Specifications
CAS Number
9007-83-4
Gene Name
Cell division cycle 25C
Gene Aliases
CDC25; CDC25 homolog C; dual specificity phosphatase CDC25C; mitosis inducer CDC25; M-phase inducer phosphatase 3; phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60.
Gene ID
995
Swiss Prot
P30307
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human CDC25C around the phosphorylation site of Ser216.
Target
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
GEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKF
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
53 kDa
Notes
Formerly GWB-ASB627
Specificity
CDC25C (Phospho-Ser216) Antibody detects endogenous levels of CDC25C only when phosphorylated at Ser216.
NCBI Gene Symbol
CDC25C
Host or Source
Rabbit
Protein Name
M-phase inducer phosphatase 3
Gene Name URL
CDC25C
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