NFAT5 Antibody (Phospho-Ser1197)
Product Specifications
CAS Number
9007-83-4
Gene Name
Nuclear factor of activated T cells 5
Gene Aliases
Glutamine rich protein H65; NF-AT5; NFATL1; NFAT-like protein 1; NFATZ; nuclear factor of activated T-cells 5; nuclear factor of activated T-cells 5, tonicity-responsive; OREBP; osmotic response element-binding protein; T-cell transcription factor NFAT5; TonE-binding protein; TONEBP; tonicity-responsive enhancer-binding protein.
Gene ID
10725
Swiss Prot
O94916
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human NFAT5 around the phosphorylation site of Ser1197.
Target
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed:10051678) . Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867) . Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
QSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPP
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
165 kDa
Notes
Formerly GWB-ASB577
Specificity
NFAT5 (Phospho-Ser1197) Antibody detects endogenous levels of NFAT5 only when phosphorylated at Ser1197.
NCBI Gene Symbol
NFAT5
Host or Source
Rabbit
Protein Name
Nuclear factor of activated T-cells 5
Gene Name URL
NFAT5
Frequently Asked Questions
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