NEK9 Antibody (Phospho-Thr210)
Product Specifications
CAS Number
9007-83-4
Gene Name
NIMA related kinase 9
Gene Aliases
APUG; LCCS10; NC; NERCC; NERCC1; nercc1 kinase; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a) - related kinase 9; NimA-related kinase 8; nimA-related protein kinase 9; serine/threonine-protein kinase Nek9.
Gene ID
91754
Swiss Prot
Q8TD19
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human NEK9 around the phosphorylation site of Thr210.
Target
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
DIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQG
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
107 kDa
Notes
Formerly GWB-ASB547
Specificity
NEK9 (Phospho-Thr210) Antibody detects endogenous levels of NEK9 only when phosphorylated at Thr210.
NCBI Gene Symbol
NEK9
Host or Source
Rabbit
Protein Name
Serine/threonine-protein kinase Nek9
Gene Name URL
NEK9
Frequently Asked Questions
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