Vinculin Antibody (Phospho-Tyr821)
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Product Specifications
CAS Number
9007-83-4
Gene Name
Vinculin
Gene Aliases
CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin; vinculin isoform meta-VCL.
Gene ID
7414
Swiss Prot
P18206
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821.
Target
Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
ASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAF
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
123 kDa
Notes
Formerly GWB-ASB471
Specificity
Vinculin (Phospho-Tyr821) Antibody detects endogenous levels of Vinculin only when phosphorylated at Tyr821.
NCBI Gene Symbol
VCL
Host or Source
Rabbit
Protein Name
Vinculin
Gene Name URL
VCL
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