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P57 Kip2 Antibody (Phospho-Thr310)

Product Specifications

CAS Number

9007-83-4

Gene Name

Cyclin dependent kinase inhibitor 1C

Gene Aliases

BWCR; BWS; cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor 1C (p57, Kip2) ; cyclin-dependent kinase inhibitor p57; KIP2; p57; p57Kip2; WBS.

Gene ID

1028

Swiss Prot

P49918

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human p57 Kip2 around the phosphorylation site of Thr310.

Target

Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

LSGPLISDFFAKRKRSAPEKSSGDVPAPCPSPSAAPGVGSVEQTPRKRLR

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Reconstitution

-20°C

Molecular Weight

32 kDa

Notes

Formerly GWB-ASB450

Specificity

P57 Kip2 (Phospho-Thr310) Antibody detects endogenous levels of p57 Kip2 only when phosphorylated at Thr310.

NCBI Gene Symbol

CDKN1C

Host or Source

Rabbit

Protein Name

Cyclin-dependent kinase inhibitor 1C

Gene Name URL

CDKN1C

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