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Histone H1 Antibody (Phospho-Thr17)

Product Specifications

CAS Number

9007-83-4

Gene Name

H1.3 linker histone, cluster member|H1.4 linker histone, cluster member|H1.5 linker histone, cluster member

Gene Aliases

DJ221C16.5; H1; H1 histone family, member 3; H1 histone family, member 4; H1 histone family, member 5; H1.3; H1.4; H1.5; H1B; H1D; H1E; H1F3; H1F4; H1F5; H1s-2; H1s-3; H1s-4; HIST1H1B; HIST1H1D; HIST1H1E; histone 1, H1b; histone 1, H1d; histone 1, H1e; histone cluster 1 H1 family member b; histone cluster 1 H1 family member d; histone cluster 1 H1 family member e; histone cluster 1, H1b; histone cluster 1, H1d; histone cluster 1, H1e; histone H1.3; histone H1.4; histone H1.5; histone H1a; histone H1b; histone H1c; histone H1s-2; histone H1s-3; histone H1s-4; RMNS.

Gene ID

3007|3008|3009

Swiss Prot

P10412|P16401|P16402

Reactivity

Human|Monkey|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17.

Target

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid.//PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

-20°C

Molecular Weight

22 kda

Notes

Formerly GWB-ASB443

Fragment

IgG

Specificity

Histone H1 (Phospho-Thr17) Antibody detects endogenous levels of Histone H1 only when phosphorylated at Thr17.

NCBI Gene Symbol

H1-3|H1-4|H1-5

Host or Source

Rabbit

Protein Name

Histone H1.3|Histone H1.4|Histone H1.5

Gene Name URL

H1-3|H1-4|H1-5

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