Rhodopsin Antibody (Phospho-Ser334)
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Product Specifications
CAS Number
9007-83-4
Gene Name
Rhodopsin
Gene Aliases
CSNBAD1; OPN2; opsin 2, rod pigment; opsin-2; rhodopsin; RP4.
Gene ID
6010
Swiss Prot
P08100
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human Rhodopsin around the phosphorylation site of Ser334.
Target
Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071) . Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452) . Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425) . Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
AIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
39 kDa
Notes
Formerly GWB-ASB219
Specificity
Rhodopsin (Phospho-Ser334) Antibody detects endogenous levels of Rhodopsin only when phosphorylated at Ser334.
NCBI Gene Symbol
RHO
Host or Source
Rabbit
Protein Name
Rhodopsin
Gene Name URL
RHO
Frequently Asked Questions
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