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Rhodopsin Antibody (Phospho-Ser334)

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Product Specifications

CAS Number

9007-83-4

Gene Name

Rhodopsin

Gene Aliases

CSNBAD1; OPN2; opsin 2, rod pigment; opsin-2; rhodopsin; RP4.

Gene ID

6010

Swiss Prot

P08100

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Rhodopsin around the phosphorylation site of Ser334.

Target

Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071) . Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452) . Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425) . Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

AIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Reconstitution

-20°C

Molecular Weight

39 kDa

Notes

Formerly GWB-ASB219

Specificity

Rhodopsin (Phospho-Ser334) Antibody detects endogenous levels of Rhodopsin only when phosphorylated at Ser334.

NCBI Gene Symbol

RHO

Host or Source

Rabbit

Protein Name

Rhodopsin

Gene Name URL

RHO

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