PP2A-alpha Antibody (Phospho-Tyr307)
Product Specifications
CAS Number
9007-83-4
Gene Name
Protein phosphatase 2 catalytic subunit alpha
Gene Aliases
NEDLBA; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; protein phosphatase 2, catalytic subunit, alpha isozyme; replication protein C; RP-C; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform.
Gene ID
5515
Swiss Prot
P67775
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307.
Target
PP2A is the major phosphatase for microtubule-associated proteins (MAPs) . PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity) . Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
FSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
35 kDa
Notes
Formerly GWB-ASB208
Specificity
PP2A-alpha (Phospho-Tyr307) Antibody detects endogenous levels of PP2A-alpha only when phosphorylated at Tyr307.
NCBI Gene Symbol
PPP2CA
Host or Source
Rabbit
Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Gene Name URL
PPP2CA
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