MYPT1 Antibody (Phospho-Thr853)

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Product Specifications

CAS Number

9007-83-4

Gene Name

Protein phosphatase 1 regulatory subunit 12A

Gene Aliases

GUBS; M130; MBS; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; MYPT1; protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit.

Gene ID

4659

Swiss Prot

O14974

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853.

Target

Key regulator of protein phosphatase 1C (PPP1C) . Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

KEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYL

Applications

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

-20°C

Molecular Weight

115 kDa

Notes

Formerly GWB-ASB180

Fragment

IgG

Specificity

MYPT1 (Phospho-Thr853) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Thr853.

NCBI Gene Symbol

PPP1R12A

Host or Source

Rabbit

Protein Name

Protein phosphatase 1 regulatory subunit 12A

Gene Name URL

PPP1R12A

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