ERF Antibody (Phospho-Thr526)
Product Specifications
CAS Number
9007-83-4
Gene Name
ETS2 repressor factor
Gene Aliases
CHYTS; CRS4; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE2; PE-2.
Gene ID
2077
Swiss Prot
P50548
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human ERF around the phosphorylation site of Thr526.
Target
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity) . May be important for regulating trophoblast stem cell differentiation (By similarity) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
CRLEGGGGPAGGFEDEGEDKKVRGEGPGEAGGPLTPRRVSSDLQHATAQL
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
58 kDa
Notes
Formerly GWB-ASB145
Specificity
ERF (Phospho-Thr526) Antibody detects endogenous levels of ERF only when phosphorylated at Thr526.
NCBI Gene Symbol
ERF
Host or Source
Rabbit
Protein Name
ETS domain-containing transcription factor ERF
Gene Name URL
ERF
Frequently Asked Questions
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