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AS160 Antibody (Phospho-Thr642)

Product Specifications

Gene Name

TBC1 domain family member 4

Gene Aliases

Akt substrate of 160 kDa; AS160; NIDDM5; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC1 domain family member 4.

Gene ID

9882

Swiss Prot

O60343

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human AS160 around the phosphorylation site of Thr642.

Target

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

PGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGR

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Reconstitution

-20°C

Molecular Weight

146 kDa

Notes

Formerly GWB-ASB130

Specificity

AS160 (Phospho-Thr642) Antibody detects endogenous levels of AS160 only when phosphorylated at Thr642.

NCBI Gene Symbol

TBC1D4

Host or Source

Rabbit

Protein Name

TBC1 domain family member 4

Gene Name URL

TBC1D4

CAS Number

9007-83-4

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