AS160 Antibody (Phospho-Thr642)
Product Specifications
CAS Number
9007-83-4
Gene Name
TBC1 domain family member 4
Gene Aliases
Akt substrate of 160 kDa; AS160; NIDDM5; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC1 domain family member 4.
Gene ID
9882
Swiss Prot
O60343
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human AS160 around the phosphorylation site of Thr642.
Target
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
PGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGR
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
146 kDa
Notes
Formerly GWB-ASB130
Specificity
AS160 (Phospho-Thr642) Antibody detects endogenous levels of AS160 only when phosphorylated at Thr642.
NCBI Gene Symbol
TBC1D4
Host or Source
Rabbit
Protein Name
TBC1 domain family member 4
Gene Name URL
TBC1D4
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