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HSL Antibody (Phospho-Ser552)

Product Specifications

CAS Number

9007-83-4

Gene Name

Lipase E, hormone sensitive type

Gene Aliases

AOMS4; FPLD6; hormone-sensitive lipase; HSL; LHS; lipase, hormone-sensitive; monoacylglycerol lipase LIPE; REH; retinyl ester hydrolase.

Gene ID

3991

Swiss Prot

Q05469

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser552.

Target

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

IDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRL

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Reconstitution

-20°C

Molecular Weight

116 kDa

Notes

Formerly GWB-ASB113

Specificity

HSL (Phospho-Ser552) Antibody detects endogenous levels of HSL only when phosphorylated at Ser552.

NCBI Gene Symbol

LIPE

Host or Source

Rabbit

Protein Name

Hormone-sensitive lipase

Gene Name URL

LIPE

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