EEF2K Antibody (Phospho-Ser366)
Product Specifications
CAS Number
9007-83-4
Gene Name
Eukaryotic elongation factor 2 kinase
Gene Aliases
Alternative protein EEF2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; CaMKIII; eEF-2 kinase; eEF-2K; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; HSU93850.
Gene ID
29904
Swiss Prot
O00418
Reactivity
Human|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366.
Target
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
RERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENS
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
-20°C
Molecular Weight
82 kDa
Notes
Formerly GWB-ASB058
Specificity
EEF2K (Phospho-Ser366) Antibody detects endogenous levels of eEF2K only when phosphorylated at Ser366.
NCBI Gene Symbol
EEF2K
Host or Source
Rabbit
Protein Name
Eukaryotic elongation factor 2 kinase
Gene Name URL
EEF2K
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