MARCH5 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Membrane associated ring-CH-type finger 5
Gene Aliases
E3 ubiquitin-protein ligase MARCH5; E3 ubiquitin-protein ligase MARCHF5; MARCH5; MARCH-V; membrane associated ring finger 5; membrane-associated ring finger (C3HC4) 5; membrane-associated RING finger protein 5; membrane-associated RING-CH protein V; mitochondrial ubiquitin ligase; MITOL; ring finger protein 153; RING-type E3 ubiquitin transferase MARCH5; RING-type E3 ubiquitin transferase MARCHF5; RNF153.
Gene ID
54708
Swiss Prot
Q9NX47
Accession Number
NP_060294.1
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human MARCH5.
Target
Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
31 kDa
Notes
Formerly GWB-ASE559
Specificity
MARCH5 antibody detects endogenous levels of total MARCH5 protein.
NCBI Gene Symbol
MARCHF5
Host or Source
Rabbit
Protein Name
E3 ubiquitin-protein ligase MARCH5
Gene Name URL
MARCHF5
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