MED21 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Mediator complex subunit 21
Gene Aliases
HSrb7; Mediator complex subunit 21; mediator of RNA polymerase II transcription subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; SRB7; SRB7 suppressor of RNA polymerase B homolog; SURB7.
Gene ID
9412
Swiss Prot
Q13503
Accession Number
NP_004255.2
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human MED21.
Target
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
15 kDa
Notes
Formerly GWB-ASD646
Specificity
MED21 antibody detects endogenous levels of total MED21 protein.
NCBI Gene Symbol
MED21
Host or Source
Rabbit
Protein Name
Mediator of RNA polymerase II transcription subunit 21
Gene Name URL
MED21
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