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TCOF1 Antibody

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Product Specifications

CAS Number

9007-83-4

Gene Name

Treacle ribosome biogenesis factor 1

Gene Aliases

MFD1; nucleolar trafficking phosphoprotein; TCS; TCS1; Treacher Collins syndrome protein; Treacher Collins-Franceschetti syndrome 1; treacle; treacle protein.

Gene ID

6949

Swiss Prot

Q13428

Accession Number

NP_000347.2

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human TCOF1.

Target

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:12777385, PubMed:26399832) . Required for neural crest specification: following monoubiquitination by the BCR (KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

152 kDa

Notes

Formerly GWB-ASD642

Specificity

TCOF1 antibody detects endogenous levels of total TCOF1 protein.

NCBI Gene Symbol

TCOF1

Host or Source

Rabbit

Protein Name

Treacle protein

Gene Name URL

TCOF1

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