SRSF3 Antibody

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Product Specifications

CAS Number

9007-83-4

Gene Name

Serine and arginine rich splicing factor 3

Gene Aliases

Epididymis secretory sperm binding protein; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; serine/arginine-rich splicing factor 3; SFRS3; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD; SRp20.

Gene ID

6428

Swiss Prot

P84103

Accession Number

NP_003008.1

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human SFRS3.

Target

Splicing factor that specifically promotes exon-inclusion during alternative splicing (PubMed:26876937) . Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A) -containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937) . Also functions as export adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244) . Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway) ; enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396) . Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244) . RNA-binding is semi-sequence specific (PubMed:17036044) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol