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TFE3 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Transcription factor binding to IGHM enhancer 3

Gene Aliases

BHLHe33; class E basic helix-loop-helix protein 33; RCCP2; RCCX1; TFEA; transcription factor E family, member A; transcription factor E3; transcription factor for IgH enhancer; transcription factor for immunoglobulin heavy-chain enhancer 3.

Gene ID

7030

Swiss Prot

P19532

Accession Number

NP_006512.2

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human TFE3.

Target

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') . Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4 (+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

61 kDa

Notes

Formerly GWB-ASD435

Specificity

TFE3 antibody detects endogenous levels of total TFE3 protein.

NCBI Gene Symbol

TFE3

Host or Source

Rabbit

Protein Name

Transcription factor E3

Gene Name URL

TFE3

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