What is the catalog number for TOP3B Antibody?

The catalog number for TOP3B Antibody is OAAF02093-100UG.

What sizes are available for TOP3B Antibody?

TOP3B Antibody is available in the following size(s): 100 µg.

Is TOP3B Antibody in stock?

TOP3B Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

TOP3B Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

DNA topoisomerase III beta

Gene Aliases

DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1; TOP3B1; topoisomerase (DNA) III beta; topoisomerase III beta.

Gene ID

8940

Swiss Prot

O95985

Accession Number

NP_003926.1

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human TOP3B.

Target

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) . Possesses negatively supercoiled DNA relaxing activity.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol