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NCK2 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

NCK adaptor protein 2

Gene Aliases

Cytoplasmic protein NCK2; GRB4; growth factor receptor-bound protein 4; NCK adaptor protein 2; NCKbeta; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta.

Gene ID

8440

Swiss Prot

O43639

Accession Number

NP_001004720.1

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human NCK2.

Target

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

42 kDa

Notes

Formerly GWB-ASD149

Specificity

NCK2 antibody detects endogenous levels of total NCK2 protein.

NCBI Gene Symbol

NCK2

Host or Source

Rabbit

Protein Name

Cytoplasmic protein NCK2

Gene Name URL

NCK2

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