NCK2 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
NCK adaptor protein 2
Gene Aliases
Cytoplasmic protein NCK2; GRB4; growth factor receptor-bound protein 4; NCK adaptor protein 2; NCKbeta; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta.
Gene ID
8440
Swiss Prot
O43639
Accession Number
NP_001004720.1
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human NCK2.
Target
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
42 kDa
Notes
Formerly GWB-ASD149
Specificity
NCK2 antibody detects endogenous levels of total NCK2 protein.
NCBI Gene Symbol
NCK2
Host or Source
Rabbit
Protein Name
Cytoplasmic protein NCK2
Gene Name URL
NCK2
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