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RapGEF1 Antibody

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Product Specifications

CAS Number

9007-83-4

Gene Name

Rap guanine nucleotide exchange factor 1

Gene Aliases

C3G; CRK SH3-binding GNRP; GRF2; Guanine nucleotide-releasing factor 2; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene) ; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; rap guanine nucleotide exchange factor 1.

Gene ID

2889

Swiss Prot

Q13905

Accession Number

NP_005303.2

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human RAPGEF1.

Target

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF) -induced sustained activation of Rap1 and neurite outgrowth.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

120 kDa

Specificity

RapGEF1 antibody detects endogenous levels of total RapGEF1 protein

NCBI Gene Symbol

RAPGEF1

Host or Source

Rabbit

Protein Name

Rap guanine nucleotide exchange factor 1

Gene Name URL

RAPGEF1

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