SPHK2 Antibody (Phospho-Thr614)
Product Specifications
CAS Number
9007-83-4
Gene Name
Sphingosine kinase 2
Gene Aliases
SK 2; SK-2; sphingosine kinase 2; SPK 2; SPK-2.
Gene ID
56848
Swiss Prot
Q9NRA0
Accession Number
NP_001191088.1
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614.
Target
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
69 kDa
Notes
Formerly GWB-ASC012
Specificity
SPHK2 (Phospho-Thr614) antibody detects endogenous levels of SPHK2 only when phosphorylated at threonine 614.
NCBI Gene Symbol
SPHK2
Host or Source
Rabbit
Protein Name
Sphingosine kinase 2
Gene Name URL
SPHK2
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