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Ephrin B1/B2/B3 Antibody (Phospho-Tyr324)

View product citations for Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520) on CiteAb View product citations for Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520) on CiteAb

Product Specifications

CAS Number

9007-83-4

Gene Name

Ephrin B1|ephrin B2|ephrin B3

Gene Aliases

CFND; CFNS; EFB1; EFL3; EFL-3; EFL6; ELK ligand; Elk-L; EPH-related receptor transmembrane ligand ELK-L3; eph-related receptor tyrosine kinase ligand 2; eph-related receptor tyrosine kinase ligand 5; eph-related receptor tyrosine kinase ligand 8; ephrin-B1; ephrin-B2; ephrin-B3; EPLG2; EPLG5; EPLG8; HTK ligand; Htk-L; HTKL; LERK2; LERK5; LERK8; ligand of eph-related kinase 5.

Gene ID

1947|1948|1949

Swiss Prot

P52799|P98172|Q15768

Accession Number

NP_001397.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.

Target

Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity) .|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

-20°C

Molecular Weight

38 kDa

Notes

Formerly GWB-ASB962

Fragment

IgG

Specificity

Ephrin B1/B2/B3 (Phospho-Tyr324) antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.

NCBI Gene Symbol

EFNB1|EFNB2|EFNB3

Host or Source

Rabbit

Protein Name

Ephrin-B1|Ephrin-B2|Ephrin-B3

Gene Name URL

EFNB1|EFNB2|EFNB3

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