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CBX5 Antibody (Phospho-Ser92)

Product Specifications

CAS Number

9007-83-4

Gene Name

Chromobox 5

Gene Aliases

Antigen p25; chromobox homolog 5 (HP1 alpha homolog, Drosophila) ; chromobox protein homolog 5; epididymis luminal protein 25; HEL25; heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HP1; HP1 alpha homolog; HP1A; HP1-ALPHA; HP1Hs alpha.

Gene ID

23468

Swiss Prot

P45973

Accession Number

NP_001120793.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human HP1 alpha around the phosphorylation site of Ser92.

Target

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) . Can interact with lamin-B receptor (LBR) . This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

22 kda

Notes

Formerly GWB-ASB957

Specificity

HP1α (Phospho-Ser92) antibody detects endogenous levels of HP1α only when phosphorylated at serine 92.

NCBI Gene Symbol

CBX5

Host or Source

Rabbit

Protein Name

Chromobox protein homolog 5

Gene Name URL

CBX5

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