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ANAPC1 Antibody (Phospho-Ser355)

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Product Specifications

CAS Number

9007-83-4

Gene Name

Anaphase promoting complex subunit 1

Gene Aliases

Anaphase-promoting complex 1 (meiotic checkpoint regulator) ; anaphase-promoting complex subunit 1; APC1; cyclosome subunit 1; MCPR; mitotic checkpoint regulator; testis-specific gene 24 protein; TSG24.

Gene ID

64682

Swiss Prot

Q9H1A4

Accession Number

NP_073153.1

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human APC1 around the phosphorylation site of Ser355.

Target

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

216 kDa

Notes

Formerly GWB-ASB950

Specificity

APC1 (Phospho-Ser355) antibody detects endogenous levels of APC1 only when phosphorylated at serine 355.

NCBI Gene Symbol

ANAPC1

Host or Source

Rabbit

Protein Name

Anaphase-promoting complex subunit 1

Gene Name URL

ANAPC1

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