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MAP3K1 Antibody (Phospho-Thr1402)

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Product Specifications

CAS Number

9007-83-4

Gene Name

Mitogen-activated protein kinase kinase kinase 1

Gene Aliases

MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK; MEKK 1; MEKK1; mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; SRXY6.

Gene ID

4214

Swiss Prot

Q13233

Accession Number

NP_005912.1

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MAP3K1 around the phosphorylation site of Thr1402.

Target

Component of a protein kinase signal transduction cascade (PubMed:9808624) . Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed:9808624) . May phosphorylate the MAPK8/JNK1 kinase (PubMed:17761173) . Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed:9808624) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Reconstitution

-20°C

Molecular Weight

161 kDa

Notes

Formerly GWB-ASB895

Fragment

IgG

Specificity

MAP3K1 (Phospho-Thr1402) antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1402.

NCBI Gene Symbol

MAP3K1

Host or Source

Rabbit

Protein Name

Mitogen-activated protein kinase kinase kinase 1

Gene Name URL

MAP3K1

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