HIRA Antibody (Phospho-Thr555)
Product Specifications
CAS Number
9007-83-4
Gene Name
Histone cell cycle regulator
Gene Aliases
DGCR1; DiGeorge critical region gene 1; HIR histone cell cycle regulation defective homolog A; histone regulator A; protein HIRA; TUP1; TUP1-like enhancer of split protein 1; TUPLE1.
Gene ID
7290
Swiss Prot
P54198
Accession Number
NP_003316.3
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human HIRA around the phosphorylation site of Thr555.
Target
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
111 kDa
Notes
Formerly GWB-ASB483
Specificity
HIRA (Phospho-Thr555) antibody detects endogenous levels of HIRA only when phosphorylated at threonine 555.
NCBI Gene Symbol
HIRA
Host or Source
Rabbit
Protein Name
Protein HIRA
Gene Name URL
HIRA
Frequently Asked Questions
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