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HIRA Antibody (Phospho-Thr555)

Product Specifications

Gene Name

Histone cell cycle regulator

Gene Aliases

DGCR1; DiGeorge critical region gene 1; HIR histone cell cycle regulation defective homolog A; histone regulator A; protein HIRA; TUP1; TUP1-like enhancer of split protein 1; TUPLE1.

Gene ID

7290

Swiss Prot

P54198

Accession Number

NP_003316.3

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human HIRA around the phosphorylation site of Thr555.

Target

Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

111 kDa

Notes

Formerly GWB-ASB483

Specificity

HIRA (Phospho-Thr555) antibody detects endogenous levels of HIRA only when phosphorylated at threonine 555.

NCBI Gene Symbol

HIRA

Host or Source

Rabbit

Protein Name

Protein HIRA

Gene Name URL

HIRA

CAS Number

9007-83-4

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