Nos1 Antibody (Phospho-Ser852)
Product Specifications
CAS Number
9007-83-4
Gene Name
Nitric oxide synthase 1
Gene Aliases
BNOS; constitutive NOS; IHPS1; NC-NOS; neuronal NOS; nitric oxide synthase 1 (neuronal) ; nitric oxide synthase, brain; nNOS; N-NOS; NOS; NOS type I; peptidyl-cysteine S-nitrosylase NOS1.
Gene ID
4842
Swiss Prot
P29475
Accession Number
NP_434686.1
Reactivity
Human|Monkey|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human n-NOS around the phosphorylation site of Ser852.
Target
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
160 kDa
Notes
Formerly GWB-ASB185
Specificity
N-NOS (Phospho-Ser852) antibody detects endogenous levels of n-NOS only when phosphorylated at serine 847.
NCBI Gene Symbol
NOS1
Host or Source
Rabbit
Protein Name
Nitric oxide synthase, brain
Gene Name URL
NOS1
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