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AKT1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

RAC-alpha serine/threonine-protein kinase

Gene Aliases

AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA

Gene ID

207

Swiss Prot

P31749

Accession Number

NP_001014431.1NP_001014432.1NP_005154.2XP_005267458.1

Reactivity

Human, Mouse, Rat, Chicken

Immunogen

Synthetic peptide corresponding to amino acids 461-477 of human PKBalpha/Akt1.

Target

PKBalphaalso knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1) . AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Clonality

Monoclonal

Clone

IML-26

Type

Monoclonal Antibody

Applications

WB

Purification

Purified by the goat anti-mouse IgG Affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Buffer

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Reconstitution

At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.

Fragment

IgG1

NCBI Gene Symbol

AKT1

Host or Source

Mouse

Protein Name

RAC-alpha serine/threonine-protein kinase

Gene Name URL

AKT1

Nucleotide Accession Number

NM_001014431.1NM_001014432.1NM_005163.2XM_005267401.1

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